Application
Fig1: Westernblot analysis of NGFR p75 expressionin SK-N-NC whole cell lysate.Application
Fig2： Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.| Product Name | NGF Recepr [2G2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD271 antibody CD271 antigen antibody Gp80 LNGFR antibody Gp80-LNGFR antibody Low affinity nerve growth factor receptor antibody Low affinity neurotrophin receptor p75NTR antibody Low-affinity nerve growth factor receptor antibody Nerve growth factor receptor antibody Nerve growth factor receptor TNFR superfamily member 16 antibody NGF receptor antibody Ngfr antibody p75 ICD antibody p75 Neurotrophin receptor antibody p75 NTR antibody p75(NTR) antibody p75NTR antibody TNFR Superfamily Member 16 antibody TNFRSF16 antibody TNR16_HUMAN antibody Tumor necrosis factor receptor superfamily member 16 antibody |
| Product description | The Trk oncogene encodes a membrane-spanning protein tyrosine kinase, gp140Trk, whose expression is restricted in vivo to neurons of the sensory spinal and cranial ganglia of neural crest origin. Nerve growth factor (NGF) stimulates tyrosine phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Tyrosine phosphorylation of Trk by NGF is rapid, specific and occurs with picomolar quantities of factor, indicating that the response is mediated by physiological amounts of NGF, suggesting that Trk A participates in the primary signal transduction mechanism of NGF. An additional component of the Trk A receptor complex, NGFR p75, binds to the neurotrophic factors with low affinity but is required for efficient signaling. NGFR p75 accelerates Trk A activation and may recruit downstream effector molecules to the liganded complex. |
| Immunogen | Amino acids 393-427 at the C-terminus of NGFR p75 of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIF |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | CD271 antibody CD271 antigen antibody Gp80 LNGFR antibody Gp80-LNGFR antibody Low affinity nerve growth factor receptor antibody Low affinity neurotrophin receptor p75NTR antibody Low-affinity nerve growth factor receptor antibody Nerve growth factor receptor antibody Nerve growth factor receptor TNFR superfamily member 16 antibody NGF receptor antibody Ngfr antibody p75 ICD antibody p75 Neurotrophin receptor antibody p75 NTR antibody p75(NTR) antibody p75NTR antibody TNFR Superfamily Member 16 antibody TNFRSF16 antibody TNR16_HUMAN antibody Tumor necrosis factor receptor superfamily member 16 antibody |
|---|---|
| Molecular Weight(MW) | 75kDa |
| Cellular Localization | Membrane |
| SwissProt ID | P08138 |
|---|
Application
Fig1: Westernblot analysis of NGFR p75 expressionin SK-N-NC whole cell lysate.
Application
Fig2： Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.| Positive Control | Hela, SK-N-NC. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
