Application
Fig1: Western blot analysis of Nrf1 expression in non-transfected Hela (A), TF-1 (B), K562 (C), NIH/3T3 (D), RPE-J (E) and 3T3-L1 (F) whole cell lysates.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human ovary tissue using anti-Nrf1 antibody. Counter stained with hematoxylin.| Product Name | NRF1 [1G1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | alpha pal antibody alpha palindromic binding protein antibody Alpha palindromic-binding protein antibody Alpha-pal antibody locus control region factor 1 antibody NFE2 related factor 1 antibody NRF-1 antibody Nrf1 antibody NRF1_HUMAN antibody Nuclear respiratory factor 1 antibody |
| Product description | The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK, and it regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (MAREs). A family of NF-E2 related proteins, which are collectively known as the Cap “n” collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, JunB and JunD) to activate gene expression, specifically the detoxifying enzyme, NQO1. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1. Nrf3 is highly expressed in placenta, B cells and monocytes. |
| Immunogen | Amino acids 191-475 of Nrf1 of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | alpha pal antibody alpha palindromic binding protein antibody Alpha palindromic-binding protein antibody Alpha-pal antibody locus control region factor 1 antibody NFE2 related factor 1 antibody NRF-1 antibody Nrf1 antibody NRF1_HUMAN antibody Nuclear respiratory factor 1 antibody |
|---|---|
| Molecular Weight(MW) | 30kDa |
| Cellular Localization | Nucleus, Endoplasmic reticulum membrane |
| SwissProt ID | Q14494 |
|---|
Application
Fig1: Western blot analysis of Nrf1 expression in non-transfected Hela (A), TF-1 (B), K562 (C), NIH/3T3 (D), RPE-J (E) and 3T3-L1 (F) whole cell lysates.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human ovary tissue using anti-Nrf1 antibody. Counter stained with hematoxylin.| Positive Control | Hela, 293T. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ ; “DO NOT FREEZE”. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
