Application
Fig1: Western blot analysis of PGP expression in human heart (A) and human skeletal muscle (B) tissue extracts and K-562 whole cell lysate (C).Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes.| Product Name | PGP [4G2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PGP antibody PGP_HUMAN antibody PGPase antibody Phosphoglycolate phosphatase antibody |
| Product description | PGP (phosphoglycolate phosphatase), also known as PGPase, is a 321 amino acid enzyme belonging to the HAD-like hydrolase superfamily and the CbbY/cbbZ/gph/yieH family. PGP is detected in all tissues including red cells, lymphocytes and cultured fibroblasts, with highest activity found in skeletal and cardiac muscle. PGP is considered an important regulatory enzyme on oxygen transport by indirectly affecting the level of red cell 2,3-diphosphoglycerate. The gene encoding PGP maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is also associated with chromosome 16, as is Crohn’s disease, which is a gastrointestinal inflammatory condition. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | PGP antibody PGP_HUMAN antibody PGPase antibody Phosphoglycolate phosphatase antibody |
|---|---|
| Molecular Weight(MW) | 34kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | A6NDG6 |
|---|
Application
Fig1: Western blot analysis of PGP expression in human heart (A) and human skeletal muscle (B) tissue extracts and K-562 whole cell lysate (C).
Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes.| Positive Control | K-562, human heart tissue, human skeletal muscle tissue, human liver tissue. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
