Application
Fig1: Western blot analysis of ERK 1/2 expression in rat cerebrum tissue extract.Application
Fig2： Immunofluorescence staining of A-431 cells showing cytoplasmic staining.| Product Name | Erk1/2 [2G1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERK 1 antibody ERK antibody ERK-2 antibody ERK1 antibody ERK2 antibody ERT1 antibody Extracellular signal regulated kinase 1 antibody Extracellular signal related kinase 1 antibody Extracellular signal-regulated kinase 2 antibody HS44KDAP antibody HUMKER1A antibody Insulin stimulated MAP2 kinase antibody MAP kinase 1 antibody MAP kinase 2 antibody MAP kinase 3 antibody MAP kinase isoform p42 antibody MAP kinase isoform p44 antibody MAPK 1 antibody MAPK 2 antibody Mapk1 antibody MAPK2 antibody MAPK3 antibody Microtubule associated protein 2 kinase antibody Mitogen activated protein kinase 3 antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 2 antibody MK01_HUMAN antibody p38 antibody p40 antibody p41 antibody p41mapk antibody p42-MAPK antibody P42MAPK antibody p44 ERK1 antibody p44 MAPK antibody P44ERK1 antibody P44MAPK antibody PRKM1 antibody PRKM2 antibody PRKM3 antibody Protein tyrosine kinase ERK2 antibody |
| Product description | Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinasekinase (MEK), MEK kinase and Raf-1. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamate-tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK 1 gene maps to chromosome 16p12-p11.2 and encodes a 379 amino acid protein that shares 83% sequence identity to ERK 2. |
| Immunogen | Amino acids 325-345 of ERK 1 of rat origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIF |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | ERK 1 antibody ERK antibody ERK-2 antibody ERK1 antibody ERK2 antibody ERT1 antibody Extracellular signal regulated kinase 1 antibody Extracellular signal related kinase 1 antibody Extracellular signal-regulated kinase 2 antibody HS44KDAP antibody HUMKER1A antibody Insulin stimulated MAP2 kinase antibody MAP kinase 1 antibody MAP kinase 2 antibody MAP kinase 3 antibody MAP kinase isoform p42 antibody MAP kinase isoform p44 antibody MAPK 1 antibody MAPK 2 antibody Mapk1 antibody MAPK2 antibody MAPK3 antibody Microtubule associated protein 2 kinase antibody Mitogen activated protein kinase 3 antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 2 antibody MK01_HUMAN antibody p38 antibody p40 antibody p41 antibody p41mapk antibody p42-MAPK antibody P42MAPK antibody p44 ERK1 antibody p44 MAPK antibody P44ERK1 antibody P44MAPK antibody PRKM1 antibody PRKM2 antibody PRKM3 antibody Protein tyrosine kinase ERK2 antibody |
|---|---|
| Molecular Weight(MW) | ERK 1: 44 kDa, ERK 2: 42 kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | P28482 |
|---|
Application
Fig1: Western blot analysis of ERK 1/2 expression in rat cerebrum tissue extract.
Application
Fig2： Immunofluorescence staining of A-431 cells showing cytoplasmic staining.| Positive Control | A-431, rat cerebrum tissue. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
