Application
Fig1: Western blot analysis of phospholamban expression in mouse heart (A) and human heart (B) tissue extracts.| Product Name | Phospholamban [1G3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cardiac phospholamban antibody CMD1P antibody CMH18 antibody PLB antibody Pln antibody PPLA_HUMAN antibody |
| Product description | The Sarco(endo)plasmic-reticulum (SER) regulatory protein, Phospholamban (PLB), is a small, plasma membrane-associated phospho-protein found in the SER of cardiac, smooth and slow-twitch muscle. Believed to assemble into a pentamer, PLB regulates cardiac contractility and Ca2+ affinity for cardiac SER Ca2+ ATPase (SERCA2a). Non-phosphorylated PLB associates with SERCA2a, and inhibits Ca2+ reuptake into the SER. PLB activation occurs when key Serine/Threonine residues in PLB (Ser 10, Ser 16, Thr 17) are phosphorylated by numerous effectors, which include PKC, PKA, PKG, and CaM kinase. Phosphorylation of PLB causes dissociation from SERCA2a and a subsequent increase in the rate of Ca2+ reuptake into the SER, which accelerates ventricular relaxation. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIF |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | Cardiac phospholamban antibody CMD1P antibody CMH18 antibody PLB antibody Pln antibody PPLA_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | Oligomer: 25 kDa, Monomer: 6 kDa |
| Cellular Localization | Sarcoplasmic reticulum membrane Mitochondrion membrane, Endoplasmic reticulum membrane |
| SwissProt ID | P26678 |
|---|
Application
Fig1: Western blot analysis of phospholamban expression in mouse heart (A) and human heart (B) tissue extracts.| Positive Control | Mouse heart tissue, human heart tissue. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
