Application
Fig1: Western blot analysis of PTEN expression in A-431 (A) and KNRK (B) whole cell lysates.Application
Fig2： Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | PTEN [4D8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 10q23del antibody BZS antibody DEC antibody GLM2 antibody MGC11227 antibody MHAM antibody MMAC1 antibody MMAC1 phosphatase and tensin homolog deleted on chromosome 10 antibody Mutated in multiple advanced cancers 1 antibody Phosphatase and tensin homolog antibody Phosphatase and tensin like protein antibody Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN antibody Pten antibody PTEN_HUMAN antibody PTEN1 antibody TEP1 antibody |
| Product description | As human tumors progress to advanced stages, one genetic alteration that occurs at high frequency is a loss of heterozygosity (LOH) at chromosome 10q23.31. Mapping of homozygous deletions on this chromosome led to the isolation of the PTEN gene, also designated MMAC1 (for mutated in multiple advanced cancers) and TEP1. This candidate tumor suppressor gene exhibits a high frequency of mutations in human glioblastomas and is also mutated in other cancers, including sporadic brain, breast, kidney and prostate cancers.PTEN has been associated with Cowden disease, an autosomal dominant cancer predisposition syndrome. The PTEN gene product is a putative protein tyrosine phosphatase that is localized to the cytoplasm, and it shares extensive homology with the cytoskeletal proteins tensin and auxilin. Gene transfer studies have indicated that the phosphatase domain of PTEN is essential for growth suppression of glioma cells. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIF |
| WB:1:1,000 IP:1-2 μg per 100-500 μg of total protein IF:1:50-500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | 10q23del antibody
BZS antibody
DEC antibody
GLM2 antibody
MGC11227 antibody
MHAM antibody
MMAC1 antibody
MMAC1 phosphatase and tensin homolog deleted on chromosome 10 antibody
Mutated in multiple advanced cancers 1 antibody
Phosphatase and tensin homolog antibody
Phosphatase and tensin like protein antibody
Phosphatidylinositol 3 4 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN antibody Pten antibody PTEN_HUMAN antibody PTEN1 antibody TEP1 antibody |
|---|---|
| Molecular Weight(MW) | 55kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | P60484 |
|---|
Application
Fig1: Western blot analysis of PTEN expression in A-431 (A) and KNRK (B) whole cell lysates.
Application
Fig2： Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Positive Control | Hela, A-431, KNRK. |
|---|---|
| Application Notes | WB:1:1,000 IP:1-2 μg per 100-500 μg of total protein IF:1:50-500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
