Application
Fig1: Western blot analysis of RBP expression in HepG2 whole cell lysate.Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing membrane staining of hepatocytes.| Product Name | RBP [4G2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cellular retinol-binding protein antibody Cellular retinol-binding protein I antibody CRBP antibody CRBP-I antibody CRBP1 antibody CRBP2 antibody RBP1 antibody RBP2 antibody RBP4 antibody RBPC antibody RET1_HUMAN antibody Retinol binding protein 1 antibody Retinol binding protein 1 cellular antibody Retinol binding protein 2 cellular antibody Retinol binding protein 4 plasma antibody Retinol-binding protein 1 antibody |
| Product description | Retinol (Vitamin A) is transported in the blood bound to its carrier protein, retinol-binding protein (RBP), also designated plasma retinol-binding protein (PRBP) or RBP4. A member of the lipocalin family, RBP conveys retinol from stores in the liver to peripheral tissues. In plasma, RBP binds transthyretin (TTR, formerly called prealbumin) to prevent glomerular filtration of low molecular weight RBP in the kidneys. The stability of this complex holds diagnostic importance because the molar ratio of RBP:TTR provides an indirect way to indicate marginal vitamin A deficiency. Vitamin A deficiency blocks the secretion of RBP resulting in defective delivery and supply to epidermal cells. Originally identified solely as a transporter protein, recent studies correlating increased levels of RBP expression in adipose tissue with insulin resistance have generated research into the possible roles the protein may play in the pathogenesis of type 2 diabetes and obesity. |
| Immunogen | Amino acids 1-201 of RBP of human origin |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Cellular retinol-binding protein antibody Cellular retinol-binding protein I antibody CRBP antibody CRBP-I antibody CRBP1 antibody CRBP2 antibody RBP1 antibody RBP2 antibody RBP4 antibody RBPC antibody RET1_HUMAN antibody Retinol binding protein 1 antibody Retinol binding protein 1 cellular antibody Retinol binding protein 2 cellular antibody Retinol binding protein 4 plasma antibody Retinol-binding protein 1 antibody |
|---|---|
| Molecular Weight(MW) | 25kDa |
| Cellular Localization | Secreted |
| SwissProt ID | P02753 |
|---|
Application
Fig1: Western blot analysis of RBP expression in HepG2 whole cell lysate.
Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing membrane staining of hepatocytes.| Positive Control | HepG2, human liver tissue |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
