Application
Fig1: Western blot analysis of SATB2 expression in KNRK (A), HT-1080 (B) and SH-SY5Y (C) nuclear extracts.| Product Name | SATB2 [4G03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA binding protein SATB2 antibody DNA-binding protein SATB2 antibody FLJ21474 antibody FLJ32076 antibody GLSS antibody KIAA1034 antibody MGC119474 antibody MGC119477 antibody SATB family member 2 antibody SATB homeobox 2 antibody SATB2 antibody SATB2_HUMAN antibody Special AT rich sequence binding protein 2 antibody Special AT-rich sequence-binding protein 2 antibody |
| Product description | SATB2 (special AT-rich sequence-binding protein 2) is a nuclear matrix protein that influences craniofacial formation mechanisms, such as jaw and palate development, and is part of a transcriptional network regulating skeletal development and osteoblast differentiation. Highly expressed in adult and fetal brain, SATB2 contains two CUT DNA-binding domains and one homeobox domain and is closely related to SATB1, a transcriptional repressor. SATB2 is thought to bind to matrix attachment regions (MARs) and regulate MAR-dependent transcription of various genes, including HoxA2 and ATF-4 (CREB-2), involved in skeletal development. Functioning as both a transcriptional activator and repressor, SATB2 can also act as a protein scaffold that can enhance the activity of other DNA-binding proteins. Defects in the gene encoding SATB2 are the cause of cleft palate manifested in conjunction with severe mental retardation. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIF |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 FC:1 μg per 1 x 106 cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | DNA binding protein SATB2 antibody DNA-binding protein SATB2 antibody FLJ21474 antibody FLJ32076 antibody GLSS antibody KIAA1034 antibody MGC119474 antibody MGC119477 antibody SATB family member 2 antibody SATB homeobox 2 antibody SATB2 antibody SATB2_HUMAN antibody Special AT rich sequence binding protein 2 antibody Special AT-rich sequence-binding protein 2 antibody |
|---|---|
| Molecular Weight(MW) | 105kDa |
| Cellular Localization | Nucleus matrix |
| SwissProt ID | Q9UPW6 |
|---|
Application
Fig1: Western blot analysis of SATB2 expression in KNRK (A), HT-1080 (B) and SH-SY5Y (C) nuclear extracts.| Positive Control | KNRK, HT-1080, SH-SY5Y |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 FC:1 μg per 1 x 106 cells |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
