Application
Fig1: Western blot analysis of SCF expression in MCF7 (A) and COLO 320DM (B) whole cell lysates and rat brain (C), mouse brain (D), human colon (E) and human lung (F) tissue extracts.Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing membrane and extracellular staining of glandular cells (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of goblet cells (B).| Product Name | SCF [4G5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C kit ligand antibody C-kit ligand antibody Ckit ligand antibody DCUA antibody DFNA69 antibody DKFZp686F2250 antibody familial progressive hyperpigmentation 2 antibody FPH2 antibody FPHH antibody KIT ligand antibody Kitl antibody KITLG antibody KL 1 antibody KL1 antibody Mast cell growth factor antibody MGF antibody MGF stem cell factor antibody SCF antibody SCF_HUMAN antibody SF antibody SHEP7 antibody sKITLG antibody Soluble KIT ligand antibody Steel factor antibody steel, mouse, homolog of antibody Stem cell factor antibody Stem cell factor precursor antibody |
| Product description | Stem cell factor (SCF) is the ligand for the transmembrane tyrosine kinase receptor proto-oncogene c-Kit. SCF, also designated KL, MGF and SLF, is a pleiotropic cytokine that has two alternatively spliced forms, 248 and 220 amino acids in length in human and mouse systems, respectively. Both the larger form and the smaller form are cleaved to produce soluble forms. The smaller splice variant lacks the proteolytic cleavage site, between amino acids 149 and 177 of the larger SCF form. The larger SCF form is expressed in fibroblasts, brain and thymus, while the smaller SCF form is found in the spleen, testis, placenta and cerebellum. The SCF ligand is essential for the development of germ cells, hematopoietic progenitor cells and melanocyte precursors. With respect to mast cells, SCF can stimulate the proliferation of mature mast cells as well as the maturation and proliferation of immature mast cells |
| Immunogen | Amino acids 26-214 of SCF of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC |
| WB:1:200-1:2,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | C kit ligand antibody
C-kit ligand antibody
Ckit ligand antibody
DCUA antibody
DFNA69 antibody
DKFZp686F2250 antibody
familial progressive hyperpigmentation 2 antibody
FPH2 antibody
FPHH antibody
KIT ligand antibody
Kitl antibody
KITLG antibody
KL 1 antibody
KL1 antibody
Mast cell growth factor antibody
MGF antibody
MGF stem cell factor antibody
SCF antibody
SCF_HUMAN antibody
SF antibody
SHEP7 antibody
sKITLG antibody
Soluble KIT ligand antibody
Steel factor antibody
steel mouse homolog of antibody Stem cell factor antibody Stem cell factor precursor antibody |
|---|---|
| Molecular Weight(MW) | 48kDa |
| Cellular Localization | Cytoplasm, Secreted, Cell membrane |
| SwissProt ID | P21583 |
|---|
Application
Fig1: Western blot analysis of SCF expression in MCF7 (A) and COLO 320DM (B) whole cell lysates and rat brain (C), mouse brain (D), human colon (E) and human lung (F) tissue extracts.
Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing membrane and extracellular staining of glandular cells (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of goblet cells (B).| Positive Control | MCF7, COLO320DM, rat brain tissue, mouse brain tissue, human colon tissue, human lung tissue, human small intestine tissue |
|---|---|
| Application Notes | WB:1:200-1:2,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
