Application
Fig1: Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid tissue showing nuclear staining of glandular cells.| Product Name | Smad2/3 [4D3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | hMAD 2 antibody hMAD 3 antibody hSMAD2 antibody hSMAD3 antibody Mad related protein 2 antibody MADH2 antibody MADH3 antibody MADR2 antibody Mothers against DPP homolog 2 antibody Mothers against DPP homolog 3 antibody Sma and Mad related protein 2 antibody SMA and MAD related protein 3 antibody SMAD 2 antibody SMAD 3 antibody SMAD family member 2 antibody SMAD family member 3 antibody smad2+3 antibody smad2/3 antibody |
| Product description | Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members.Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC |
| WB:1:1,000 IP:1-2 μg per 100-500 μg of total protein IF:1:50-500 IHC:1:50-500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | hMAD 2 antibody hMAD 3 antibody hSMAD2 antibody hSMAD3 antibody Mad related protein 2 antibody MADH2 antibody MADH3 antibody MADR2 antibody Mothers against DPP homolog 2 antibody Mothers against DPP homolog 3 antibody Sma and Mad related protein 2 antibody SMA and MAD related protein 3 antibody SMAD 2 antibody SMAD 3 antibody SMAD family member 2 antibody SMAD family member 3 antibody smad2+3 antibody smad2/3 antibody |
|---|---|
| Molecular Weight(MW) | 55-60kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | Q15796 |
|---|
Application
Fig1: Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid tissue showing nuclear staining of glandular cells.| Positive Control | human thyroid tissue. |
|---|---|
| Application Notes | WB:1:1,000 IP:1-2 μg per 100-500 μg of total protein IF:1:50-500 IHC:1:50-500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
