Application
Fig1: Western blot analysis of YAP expression in non-transfected 293T (A), human YAP transfected 293T (B) and Caco-2 (C) whole cell lysates.| Product Name | YAP1 [3G1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 65 kDa Yes associated protein antibody 65 kDa Yes-associated protein antibody COB1 antibody YAp 1 antibody YAP 65 antibody YAP antibody YAP1 antibody YAP1_HUMAN antibody YAP2 antibody YAP65 antibody yes -associated protein delta antibody Yes associated protein 1 65kDa antibody Yes associated protein 1 antibody Yes associated protein 2 antibody yes associated protein beta antibody YKI antibody Yorkie homolog antibody |
| Product description | The Yes-associated protein, otherwise known as YAP, is a 14-3-3-bindingmolecule that was originally recognized by virtue of its ability to bind to theSH3 domain of Yes. The binding of YAP to 14-3-3 requires the phosphorylationof a homologous serine residue (Ser 112) in the YAP 14-3-3-binding motif. Thehighly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation,cell cycle progression and apoptosis by binding intracellular phosphoproteinsinvolved in signal transduction. YAP may link events at the plasmamembrane and cytoskeleton to inhibition of transcription in the nucleus in amanner regulated by 14-3-3 proteins. YAP shares homology with the WWdomain of TAZ, transcriptional co-activator with PDZ-binding motif, whichfunctions as a transcriptional co-activator by binding to the PPXY motif presentin transcription factors. YAP is expressed at high levels in the lung, placenta,prostate, ovary and testis. |
| Immunogen | Amino acids 294-323 within an internal region of YAP of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIF |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | 65 kDa Yes associated protein antibody 65 kDa Yes-associated protein antibody COB1 antibody YAp 1 antibody YAP 65 antibody YAP antibody YAP1 antibody YAP1_HUMAN antibody YAP2 antibody YAP65 antibody yes -associated protein delta antibody Yes associated protein 1 65kDa antibody Yes associated protein 1 antibody Yes associated protein 2 antibody yes associated protein beta antibody YKI antibody Yorkie homolog antibody |
|---|---|
| Molecular Weight(MW) | 65kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | P46937 |
|---|
Application
Fig1: Western blot analysis of YAP expression in non-transfected 293T (A), human YAP transfected 293T (B) and Caco-2 (C) whole cell lysates.| Positive Control | 293T, Caco-2 |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
