Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using ADORA2A mouse mAb against CHO3D10(1),COS7(2),F9(3),L1210(4),C6(5),C2C12(6),NIH/3T3(7),Raw264.7(8) cell lysate.Application
Figure 3:Western blot analysis using ADORA2A mAb against HEK293 (1) and ADORA2A (AA: 274-412)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Immunofluorescence analysis of Hela cells using ADORA2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 5:Flow cytometric analysis of Hela cells using ADORA2A mouse mAb (green) and negative control (red).| Product Name | ADORA2A |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human ADORA2A (AA: 274-412) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | FCMICCICC/IFWB |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | A2aR RDC8 ADORA2 |
|---|---|
| Molecular Weight(MW) | 44.7kDa |
| Entrez Gene | 135 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using ADORA2A mouse mAb against CHO3D10(1),COS7(2),F9(3),L1210(4),C6(5),C2C12(6),NIH/3T3(7),Raw264.7(8) cell lysate.
Application
Figure 3:Western blot analysis using ADORA2A mAb against HEK293 (1) and ADORA2A (AA: 274-412)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Immunofluorescence analysis of Hela cells using ADORA2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 5:Flow cytometric analysis of Hela cells using ADORA2A mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
