Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Application
Figure 2:Western blot analysis using TLR9 mAb against human TLR9 (AA: 868-1016) recombinant protein. (Expected MW is 43.4 kDa)Application
Figure 3:Western blot analysis using TLR9 mAb against HEK293 (1) and TLR9 (AA: 868-1016)-hIgGFc transfected HEK293 (2) cell lysate.Application
Figure 4:Immunofluorescence analysis of SK-OV-3 cells using TLR9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)Application
Figure 5:Flow cytometric analysis of A549 cells using TLR9 mouse mAb (green) and negative control (red).Application
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TLR9 mouse mAb with DAB staining.Application
Figure 7:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using TLR9 mouse mAb with DAB staining.| Product Name | TLR9 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human TLR9 (AA: 868-1016) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | FCMICCICC/IFIHCWB |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | CD289 |
|---|---|
| Molecular Weight(MW) | 115.8kDa |
| Entrez Gene | 54106 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using TLR9 mAb against human TLR9 (AA: 868-1016) recombinant protein. (Expected MW is 43.4 kDa)
Application
Figure 3:Western blot analysis using TLR9 mAb against HEK293 (1) and TLR9 (AA: 868-1016)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Immunofluorescence analysis of SK-OV-3 cells using TLR9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 5:Flow cytometric analysis of A549 cells using TLR9 mouse mAb (green) and negative control (red).
Application
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TLR9 mouse mAb with DAB staining.
Application
Figure 7:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using TLR9 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
