WB
Western blot analysis using ZO 1 antibody against A431 (1) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissues using ZO 1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using ZO 1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using ZO 1 antibody with DAB staining.WB
Western blot analysis using ZO 1 antibody against Rat intestine (1),Rat stomach(2) tissues lysate.WB
Comparison of the expressions of duodenal tight junction ligandins ZO-1 and occludin, and the adherens ligandin β-catenin in all the groups (mean ± SD) Notes: *P < 0.05, **P < 0.01, ***P < 0.001. There were six rats in each group (6/group).| Product Name | Anti ZO 1 antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TJP1 |
|---|---|
| Gene Synonyms | Tight junction protein 1 Tight junction protein ZO 1 TJP1 ZO 1 ZO1 ZO-1 Zona occludens protein 1 Zonula occludens protein 1 |
| Gene Full Name | tight junction protein 1 |
| Gene Summary | This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017] |
| Molecular Weight(MW) | 195 kDa |
| Source | Rabbit |
| Cellular Localization | Cell junction; Cell membrane; Cell projection; Gap junction; Membrane; Tight junction |
| SwissProt ID | Q07157 |
|---|
WB
Western blot analysis using ZO 1 antibody against A431 (1) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissues using ZO 1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using ZO 1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using ZO 1 antibody with DAB staining.
WB
Western blot analysis using ZO 1 antibody against Rat intestine (1),Rat stomach(2) tissues lysate.
WB
Comparison of the expressions of duodenal tight junction ligandins ZO-1 and occludin, and the adherens ligandin β-catenin in all the groups (mean ± SD) Notes: *P < 0.05, **P < 0.01, ***P < 0.001. There were six rats in each group (6/group).| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
