WB
Various lysates were subjected to SDS PAGE followed by western blot with ZO-1 Monoclonal antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using ZO-1 Monoclonal antibody at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ZO-1 Monoclonal antibody at dilution of 1:400 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-1 Monoclonal antibody at dilution of 1:1500 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (red).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using ZO-1 Monoclonal antibody at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).| Product Name | ZO-1 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ZO1 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:1000-1:6000 IHC :1:200-1:800 IF-P :1:200-1:800 ICC/IF:1:400-1:1600 |
|
| Species Reactivity | CanineHuman |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | TJP1 |
|---|---|
| Gene Synonyms | ZO-1 |
| Gene Full Name | tight junction protein 1 |
| Gene Summary | This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017] |
| Alternative Names | TJP1 ZO 1 Tight junction protein ZO-1 Tight junction protein ZO 1 Tight junction protein 1 |
| Molecular Weight(MW) | 1748 aa, 195 kDa(Observed band size: 230 kDa) |
| Cellular Localization | Cell junction,Cell membrane,Cell projection,Gap junction,Membrane,Tight junction |
WB
Various lysates were subjected to SDS PAGE followed by western blot with ZO-1 Monoclonal antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using ZO-1 Monoclonal antibody at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ZO-1 Monoclonal antibody at dilution of 1:400 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-1 Monoclonal antibody at dilution of 1:1500 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (red).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using ZO-1 Monoclonal antibody at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).| Application Notes | WB:1:1000-1:6000 IHC :1:200-1:800 IF-P :1:200-1:800 ICC/IF:1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
