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Anti-Nestin antibody
Catalog Number:OM641945
OM641945
Discount Price
OM641945-100ul
1~2 Week
100ul
OM641945-50ul
50ul
OM641945-20ul
1~2 Week
20ul
1~2 Week
Product Profile
Product Name Anti-Nestin antibody
Antibody Type Primary Antibodies
Immunogen Polypeptide
Key Feature
Clonality polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications ELISAICC/IFWB
WB:1:200-1:2000
ICC/IF:1:100-1:500
Species Reactivity HumanMouseRat
Concentration 1mg/ml
Purification Protein A
Target Information
Gene Symbol NES
Gene Synonyms NES
Nbla00170
NEST
Gene Full Name nestin
Gene Summary This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]
Molecular Weight(MW) 177 kDa(Observed:110 kDa)
Source Rabbit
Cellular Localization Intermediate filament
Application

WB

Western blot analysis using Nestin antibody against A431 (1), COS-7 (2), HepG2 (3) cell lysate.12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.

ICC/IF

Immunofluorescence analysis of NIH3T3 cells using Nestin antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
Application Notes WB:1:200-1:2000
ICC/IF:1:100-1:500
Additional Information
Form Liquid
Storage Instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer Purified antibody in PBS with 0.05% sodium azide.
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