WB
Western blot analysis using CD4 antibody against MCF-7(1),CaCo-2(2)cell lysate.12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ICC/IF
Immunofluorescence analysis of A549 cells using CD4 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.ICC/IF
IDO and CD4 immunofluorescence staining of wounds in mice on the 5th and 7th days post-transplantation. Note: Green represents IDO and red represents CD4. n = 5, the difference was statistically significant (***p < 0.001). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-CD4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB:1:1000-1:2000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CD4 |
|---|---|
| Gene Synonyms | CD3-DELTA CD3-GAMMA CD3-ZETA CD3DELTA CD3epsilon CD3GAMMA CD3H CD3Q CD3Z CD3ZETA CD4mut IMD17 IMD18 IMD19 IMD25 IMD79 Leu-3 OKT4D T3D T3E T3G T3Z T4 TCRE TCRZ |
| Gene Full Name | CD4 molecule |
| Gene Summary | This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. |
| Alternative Names | CD247 CD247 antigen zeta subunit CD3 antigen delta subunit CD3 delta CD3-epsilon CD3d antigen delta polypeptide (TiT3 complex) CD3d molecule delta (CD3-TCR complex) CD3e antigen epsilon polypeptide (TiT3 complex) CD3e molecule epsilon (CD3-TCR complex) CD3g antigen gamma polypeptide (TiT3 complex) CD3g molecule epsilon (CD3-TCR complex) CD3g molecule gamma (CD3-TCR complex) CD3Z antigen zeta polypeptide (TiT3 complex) CD3zeta chain CD4 antigen (p55) CD4 antigen p55 CD4 receptor component of the TCR/CD3 complex FLJ18683 Leu-2 MAL OKT3 delta chain OKT8 T-cell antigen T cell co-receptor T-cell antigen Leu2 T-cell antigen receptor complex epsilon subunit of T3 T-cell antigen receptor complex gamma subunit of T3 T-cell antigen receptor complex zeta subunit of CD3 T-cell receptor T3 delta chain T-cell receptor T3 gamma chain T-cell receptor T3 zeta chain T-cell surface antigen T3/Leu-4 epsilon chain T-cell surface antigen T4/Leu-3 T4 surface glycoprotein precursor T8 T-cell antigen TCR zeta TCR zeta chain tissue factor-targeting CAR1 dimer tissue factor-targeting CAR1 monomer |
| Molecular Weight(MW) | 51 kDa |
| Cellular Localization | Plasma membrane |
| SwissProt ID | P01730 |
|---|
WB
Western blot analysis using CD4 antibody against MCF-7(1),CaCo-2(2)cell lysate.12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ICC/IF
Immunofluorescence analysis of A549 cells using CD4 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
ICC/IF
IDO and CD4 immunofluorescence staining of wounds in mice on the 5th and 7th days post-transplantation. Note: Green represents IDO and red represents CD4. n = 5, the difference was statistically significant (***p < 0.001). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
