WB
Western blot analysis of extracts from 293 cells(UV treatment), using Phospho-STAT2 (Thr387) Ab. The lane on the left was treated with blocking peptide.IHC
Phospho-STAT2 (Thr387) Ab at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.ICC
Phospho-STAT2 (Thr387) Ab AF3653 staining A549 cells(heat shock treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF3653) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).| Product Name | Phospho-STAT2 (Thr387) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. Acts as a regulator of mitochondrial fission by modulating the phosphorylation of DNM1L at 'Ser-616' and 'Ser-637' which activate and inactivate the GTPase activity of DNM1L respectively. |
| Immunogen | A synthesized peptide derived from human STAT2 around the phosphorylation site of Thr387. |
| Modification | p-Thr387 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STAT2(6773) |
|---|---|
| Gene Synonyms | P113 IMD44 ISGF-3 PTORCH3 STAT113 |
| Gene Full Name | STAT2 signal transducer and activator of transcription 2 [ Homo sapiens (human) ] |
| Gene Summary | the protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. The protein mediates innate antiviral activity. Mutations in this gene result in Immunodeficiency 44. [provided by RefSeq, Aug 2020] |
| Target | Phospho-STAT2 (Thr387) Antibody detects endogenous levels of STAT2 only when phosphorylated at Thr387. |
| Target Description | Tyrosine phosphorylated in response to IFN-alpha. Phosphorylation at Ser-287 negatively regulates the transcriptional response |
| Molecular Weight(MW) | 98kD(Calculated) |
| Domain | Belongs to the transcription factor STAT family. |
| Cellular Localization | Cytoplasm. Nucleus. |
WB
Western blot analysis of extracts from 293 cells(UV treatment), using Phospho-STAT2 (Thr387) Ab. The lane on the left was treated with blocking peptide.
IHC
Phospho-STAT2 (Thr387) Ab at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.
ICC
Phospho-STAT2 (Thr387) Ab AF3653 staining A549 cells(heat shock treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF3653) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol |
