WB
Western Blot analysis using DDX4 Monoclonal Antibody against DDX4-hIgGFc transfected HEK293 cell lysate.IHC
Immunohistochemistry analysis of paraffin-embedded human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization with DAB staining using DDX4 Monoclonal Antibody.ICC/IF
Immunofluorescence analysis of MSCs(left) and NTERA-2 (right) cells using DDX4 Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.FCM
Flow cytometric analysis of MSCS cells using DDX4 Monoclonal Antibody (green) and negative control (purple).| Product Name | DDX4 Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human DDX4 expressed in E. Coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAFCMICC/IFIHCWB |
| WB:1:500 - 1:2000 IHC:1:200 - 1:1000 IF:1:200 - 1:1000 FCM:1:200 - 1:400 ELISA:1:10000 |
|
| Species Reactivity | Human |
| Purification | Affinity purified |
| Gene Symbol | DDX4 |
|---|---|
| Gene Synonyms | VASA |
| Gene Full Name | DEAD-box helicase 4 |
| Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| Molecular Weight(MW) | 79kDa |
| Cellular Localization | Cytoplasm . Cytoplasm, perinuclear region . Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. . |
WB
Western Blot analysis using DDX4 Monoclonal Antibody against DDX4-hIgGFc transfected HEK293 cell lysate.
IHC
Immunohistochemistry analysis of paraffin-embedded human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization with DAB staining using DDX4 Monoclonal Antibody.
ICC/IF
Immunofluorescence analysis of MSCs(left) and NTERA-2 (right) cells using DDX4 Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
FCM
Flow cytometric analysis of MSCS cells using DDX4 Monoclonal Antibody (green) and negative control (purple).| Application Notes | WB:1:500 - 1:2000 IHC:1:200 - 1:1000 IF:1:200 - 1:1000 FCM:1:200 - 1:400 ELISA:1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
