WB
Western blot analysis of lysates from Hep G2 cells using eRF3A/GSPT1 Rabbit mAb at 1:10000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:1s.IHC
Immunohistochemistry analysis of eRF3A/GSPT1 in paraffin-embedded Rat liver tissue using eRF3A/GSPT1 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.ICC/IF
Confocal imaging of Hep G2 cells using eRF3A/GSPT1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). objective: 100x.| Product Name | eRF3A/GSPT1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of eRF3A/GSPT1 (NP_001123479.1). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1:500-1:1000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GSPT1 |
|---|---|
| Gene Synonyms | GST1 ETF3A eRF3a 551G9.2 |
| Gene Full Name | G1 to S phase transition 1 |
| Gene Summary | Enables translation release factor activity. Involved in regulation of translational termination. Acts upstream of or within protein methylation. Predicted to be located in cytosol. Predicted to be part of translation release factor complex. [provided by Alliance of Genome Resources, Apr 2022] |
| Alternative Names | GST1 ETF3A eRF3a 551G9.2 |
| Molecular Weight(MW) | 56kDa(Observed MW 80kDa) |
| Cellular Localization | cytoplasm,cytosol,translation release factor complex |
WB
Western blot analysis of lysates from Hep G2 cells using eRF3A/GSPT1 Rabbit mAb at 1:10000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:1s.
IHC
Immunohistochemistry analysis of eRF3A/GSPT1 in paraffin-embedded Rat liver tissue using eRF3A/GSPT1 Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ICC/IF
Confocal imaging of Hep G2 cells using eRF3A/GSPT1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). objective: 100x.| Application Notes | WB:1:2000-1:10000 IHC:1:500-1:1000 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
