WB
Western blot analysis of GSPT1/2 using anti-GSPT1/2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: K562 whole cell lysates, Lane 2: Raji whole cell lysates, Lane 3: rat pancreas tissue lysates, Lane 4: mouse pancreas tissue lysates, Lane 5: NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GSPT1/2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of GSPT1/2 using anti-GSPT1/2 antibody. GSPT1/2 was detected in a paraffin-embedded section of human Gall bladder adenosquamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GSPT1/2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of GSPT1/2 using anti-GSPT1/2 antibody. GSPT1/2 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-GSPT1/2 Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of Jurkat cells using anti-GSPT1/2 antibody. Overlay histogram showing Jurkat cells stained with anti-GSPT1/2 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GSPT1/2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-GSPT1/2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human GSPT1,2 recombinant protein (Position: K138-D628). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GSPT1 |
|---|---|
| Gene Synonyms | GST1 ETF3A eRF3a 551G9.2 |
| Gene Full Name | G1 to S phase transition 1 |
| Gene Summary | Enables GTPase activity and translation release factor activity. Involved in regulation of translational termination and translational termination. Acts upstream of or within protein methylation. Part of translation release factor complex. Is active in cytosolic ribosome. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | ERF3A GSPT1 ERF3B GSPT2 eRF3b |
| Molecular Weight(MW) | 56/69kDa(Observed MW 85kDa) |
| Cellular Localization | cytoplasm |
WB
Western blot analysis of GSPT1/2 using anti-GSPT1/2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: K562 whole cell lysates, Lane 2: Raji whole cell lysates, Lane 3: rat pancreas tissue lysates, Lane 4: mouse pancreas tissue lysates, Lane 5: NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GSPT1/2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of GSPT1/2 using anti-GSPT1/2 antibody. GSPT1/2 was detected in a paraffin-embedded section of human Gall bladder adenosquamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GSPT1/2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of GSPT1/2 using anti-GSPT1/2 antibody. GSPT1/2 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-GSPT1/2 Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of Jurkat cells using anti-GSPT1/2 antibody. Overlay histogram showing Jurkat cells stained with anti-GSPT1/2 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GSPT1/2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
