WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-eRF3a antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human colon was stained with anti-eRF3a Rabbit antibody.| Product Name | eRF3a Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GSPT1 |
|---|---|
| Gene Synonyms | GST1 ETF3A eRF3a 551G9.2 |
| Gene Full Name | G1 to S phase transition 1 |
| Gene Summary | Enables GTPase activity and translation release factor activity. Involved in regulation of translational termination and translational termination. Acts upstream of or within protein methylation. Part of translation release factor complex. Is active in cytosolic ribosome. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | GSPT1 ERF3A Eukaryotic peptide chain release factor GTP-binding subunit ERF3A Eukaryotic peptide chain release factor subunit 3a eRF3a G1 to S phase transition protein 1 homolog |
| Molecular Weight(MW) | 56 kDa(Observed band size: 80 kDa) |
| Cellular Localization | intracellular ,cytosol ,translation release factor complex . |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-eRF3a antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human colon was stained with anti-eRF3a Rabbit antibody.| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
