WB
Western blot analysis on HT29 cell lysates using GPRIN1 Antibody,The lane on the left was treated with the antigen-specific peptide.ICC/IF
GPRIN1 Antibody staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | Rabbit polyclonal antibody to GPRIN1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human GPRIN1(Accession Q7Z2K8), corresponding to amino acid residues K232-D282. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| [WB:1:500-1:2000 ICC:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPRIN1 |
|---|---|
| Gene Synonyms | GRIN1 |
| Gene Full Name | G protein regulated inducer of neurite outgrowth 1 |
| Gene Summary | Predicted to enable phosphoprotein binding activity. Predicted to be involved in neuron projection development. Predicted to be located in growth cone. Predicted to be active in plasma membrane. [provided by Alliance of Genome Resources, Nov 2024] |
| Alternative Names | G protein regulated inducer of neurite outgrowth 1 G protein-regulated inducer of neurite outgrowth 1 GPRIN1 GRIN1 GRIN1_HUMAN KIAA1893 Z16 |
| Molecular Weight(MW) | 102kDa; 102kD(Calculated). |
| Cellular Localization | Cell membrane>Lipid-anchor. Cell projection>Growth cone. Note: Highly enriched in growth cone. |
WB
Western blot analysis on HT29 cell lysates using GPRIN1 Antibody,The lane on the left was treated with the antigen-specific peptide.
ICC/IF
GPRIN1 Antibody staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | [WB:1:500-1:2000 ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
