WB
Anti-SYVN1/HRD1 antibody at 1/1000 dilution Lane 1 : SH-SY5Y cell lysate Lane 2 : 293T cell lysate Lane 3 : Ramos cell lysate Lane 4 : HeLa cell lysate Lane 5 : HepG2 cell lysate Lysates/proteins at 10ug per lane. Secondary All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution. Developed using the ECL technique. Predicted band size: 67 kDaICC/IF
Immunofluorescence analysis of HeLa cells labeling SYVN1/HRD1, using Anti-SYVN1/HRD1 antibody at a 1/100 dilution (red), and DAPI nuclear staining (blue).FC
Intracellular Flow Cytometry analysis of permeabilized 293T cells labeling SYVN1/HRD1 (red), using Anti-SYVN1/HRD1 antibody at a 1/10 dilution, or a negative control rabbit IgG (green).| Product Name | Rabbit monoclonal to SYVN1/HRD1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human SYVN1/HRD1 aa 150-250 (internal sequence) (Cysteine residue). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:1000-1:10000 ICC:1:100-1:250 FC:1:10-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SYVN1 |
|---|---|
| Gene Synonyms | DER3 HRD1 |
| Gene Full Name | synoviolin 1 |
| Gene Summary | This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. Sequence analysis identified two transcript variants that encode different isoforms. [provided by RefSeq, May 2011] |
| Alternative Names | HRD1 KIAA1810 SYVN1 E3 ubiquitin-protein ligase synoviolin RING-type E3 ubiquitin transferase synoviolin Synovial apoptosis inhibitor 1 |
| Molecular Weight(MW) | 67kDa |
| Cellular Localization | Endoplasmic reticulum membrane. |
WB
Anti-SYVN1/HRD1 antibody at 1/1000 dilution Lane 1 : SH-SY5Y cell lysate Lane 2 : 293T cell lysate Lane 3 : Ramos cell lysate Lane 4 : HeLa cell lysate Lane 5 : HepG2 cell lysate Lysates/proteins at 10ug per lane. Secondary All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution. Developed using the ECL technique. Predicted band size: 67 kDa
ICC/IF
Immunofluorescence analysis of HeLa cells labeling SYVN1/HRD1, using Anti-SYVN1/HRD1 antibody at a 1/100 dilution (red), and DAPI nuclear staining (blue).
FC
Intracellular Flow Cytometry analysis of permeabilized 293T cells labeling SYVN1/HRD1 (red), using Anti-SYVN1/HRD1 antibody at a 1/10 dilution, or a negative control rabbit IgG (green).| Application Notes | WB:1:1000-1:10000 ICC:1:100-1:250 FC:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | pH: 7.20, Preservative: 0.01% Sodium azide. Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant. |
