WB
Various lysates were subjected to SDS PAGE followed by western blot with Nectin-3/PVRL3 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human prostate cancer using Nectin-3/PVRL3 antibody at dilution of 1:100 (under 10x lens).IP
IP result of anti-Nectin-3/PVRL3 (IP:4ug; Detection: 1:600) with mouse testis tissue lysate 4000ug.| Product Name | Nectin-3/PVRL3 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | PVRL3, Nectin 3 Fusion Protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:1000-1:4000 IHC:1:20-1:200 ICC:0.5-4.0ug for 1.0-3.0mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.75mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NECTIN3 |
|---|---|
| Gene Synonyms | PPR3 PRR3 CD113 PVRL3 PVRR3 CDW113 NECTIN-3 |
| Gene Full Name | nectin cell adhesion molecule 3 |
| Gene Summary | This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011] |
| Alternative Names | Nectin-3 PVRL3 CD113 Nectin cell adhesion molecule 3 poliovirus receptor related 3 |
| Molecular Weight(MW) | 61kDa(Observed Molecular Weight:70-80kDa) |
| Cellular Localization | Cell membrane,Postsynaptic cell membrane,Cell junction,adherens junction. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with Nectin-3/PVRL3 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human prostate cancer using Nectin-3/PVRL3 antibody at dilution of 1:100 (under 10x lens).
IP
IP result of anti-Nectin-3/PVRL3 (IP:4ug; Detection: 1:600) with mouse testis tissue lysate 4000ug.| Application Notes | WB:1:1000-1:4000 IHC:1:20-1:200 ICC:0.5-4.0ug for 1.0-3.0mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
