WB
Western blot analysis of 5-methylcytosine (5-mC) on different conjugations with Mouse anti-5-methylcytosine (5-mC) antibody at 1/1,000 dilution. Lane 1: Uridine-BSA (negative) Lane 2: Cytidine-BSA (negative) Lane 3: 5-Methylcytosine-BSA (positive) Lane 4: 3-Methylcytosine-BSA (negative) Lane 5: 5-Hydroxymethylcytidine-BSA (negative) Lysates/proteins at 2 μg/Lane. Exposure time: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-5-methylcytosine (5-mC) antibody at 1/200 dilution. The section was denatured in 2M HCl for 15 minutes at room temperature, then neutralized in 0.1M Tris-HCl (pH 8.3) for 10 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | 5-methylcytosine (5-mC) Recombinant Mouse Monoclonal Antibody |
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| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein fused with 5-methylcytosine. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | A wide range of other species |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | 5 m C antibody 5 mC antibody 5 me C antibody 5 Me Cytidine antibody 5 Me Cytosine antibody 5 meC antibody 5 Methycytosine antibody 5 Methyl Cytidine antibody 5 Methyl Cytosine antibody 5 Methyl-Cytidine antibody 5 m C antibody 5 mC antibody 5 me C antibody 5 Me Cytidine antibody 5 Me Cytosine antibody 5 meC antibody 5 Methycytosine antibody 5 Methyl Cytidine antibody 5 Methyl Cytosine antibody 5 Methyl-Cytidine antibody 5 Methyl-Cytosine antibody 5 MethylCytidine antibody 5 MethylCytosine antibody 5-mC antibody 5-Me Cytidine antibody 5-Me Cytosine antibody 5-meC antibody 5-Methyl Cytidine antibody 5-Methyl-Cytidine antibody 5-Methyl-Cytosine antibody 5-MethylCytidine antibody 5-MethylCytosine antibody 5mC antibody methyl CpG antibody |
|---|---|
| Molecular Weight(MW) | 66kDa |
| Function | 5-Methylcytosine is a methylated form of the DNA base cytosine (C) that regulates gene transcription and takes several other biological roles. When cytosine is methylated, the DNA maintains the same sequence, but the expression of methylated genes can be altered (the study of this is part of the field of epigenetics). 5-Methylcytosine is incorporated in the nucleoside 5-methylcytidine. In 5-methylcytosine, a methyl group is attached to the 5th atom in the 6-atom ring, counting counterclockwise from the NH-bonded nitrogen at the six o'clock position. This methyl group distinguishes 5-methylcytosine from cytosine. |
WB
Western blot analysis of 5-methylcytosine (5-mC) on different conjugations with Mouse anti-5-methylcytosine (5-mC) antibody at 1/1,000 dilution. Lane 1: Uridine-BSA (negative) Lane 2: Cytidine-BSA (negative) Lane 3: 5-Methylcytosine-BSA (positive) Lane 4: 3-Methylcytosine-BSA (negative) Lane 5: 5-Hydroxymethylcytidine-BSA (negative) Lysates/proteins at 2 μg/Lane. Exposure time: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-5-methylcytosine (5-mC) antibody at 1/200 dilution. The section was denatured in 2M HCl for 15 minutes at room temperature, then neutralized in 0.1M Tris-HCl (pH 8.3) for 10 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
