WB
Western blot analysis of FLAP on different lysates with Rabbit anti-FLAP antibody at 1/1,000 dilution. Lane 1: U-937 cell lysate, Lane 2: THP-1 cell lysate, Lane 3: THP-1 treated with 50nM TPA overnight then add 5μg/mL LPS for 8 hours cell lysate. Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | FLAP Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human FLAP aa 100-161. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ALOX5AP |
|---|---|
| Gene Synonyms | FLAP |
| Gene Full Name | arachidonate 5-lipoxygenase activating protein |
| Gene Summary | This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2011] |
| Alternative Names | 5-lipoxygenase activating protein antibody AL5AP_HUMAN antibody ALOX 5AP antibody ALOX5 AP antibody ALOX5AP antibody Arachidonate 5 lipoxygenase activating protein antibody Arachidonate 5-lipoxygenase-activating protein antibody Five lipoxygenase activating protein antibody FLAP antibody MK 886 binding protein antibody 5-lipoxygenase activating protein antibody AL5AP_HUMAN antibody ALOX 5AP antibody ALOX5 AP antibody ALOX5AP antibody Arachidonate 5 lipoxygenase activating protein antibody Arachidonate 5-lipoxygenase-activating protein antibody Five lipoxygenase activating protein antibody FLAP antibody MK 886 binding protein antibody MK-886-binding protein antibody |
| Molecular Weight(MW) | 18kDa |
| Cellular Localization | Nucleus membrane, Endoplasmic reticulum membrane. |
WB
Western blot analysis of FLAP on different lysates with Rabbit anti-FLAP antibody at 1/1,000 dilution. Lane 1: U-937 cell lysate, Lane 2: THP-1 cell lysate, Lane 3: THP-1 treated with 50nM TPA overnight then add 5μg/mL LPS for 8 hours cell lysate. Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
