IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 Bcl-XL antibody at dilution of 1:200.FC
1X10^6 Jurkatcells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Bcl-XL (red), or 0.4 ug CoraLite® Plus 555Mouse IgG2b Isotype Control (MPC-11) (blue). Cells were fixedwith 4%PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | CoraLite®555-conjugated Bcl-XL Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. |
| Clonality | Monoclonal |
|---|---|
| Isotype | Ig2b |
| Host Species | Mouse |
| Tested Applications | FCICC/IF |
| FC:1:50-1:500 ICC:1:50-1:200 |
|
| Species Reactivity | CanineHuman |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BCL2L1 |
|---|---|
| Gene Synonyms | BCLX BCL2L Bcl-X PPP1R52 BCL-XL/S |
| Alternative Names | Bcl-xL 4C12A6 Apoptosis regulator Bcl X Apoptosis regulator Bcl-X Bcl2-L-1 |
| Molecular Weight(MW) | 26 |
IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 Bcl-XL antibody at dilution of 1:200.
FC
1X10^6 Jurkatcells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Bcl-XL (red), or 0.4 ug CoraLite® Plus 555Mouse IgG2b Isotype Control (MPC-11) (blue). Cells were fixedwith 4%PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | FC:1:50-1:500 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
