WB
Various lysates were subjected to SDS PAGE followed by western blot with NRF2, NFE2L2 antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin embedded human liver cancer tissue slide using NRF2, NFE2L2 Antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NRF2, NFE2L2 antibody at dilution of 1:50 and CoraLite488 Conjugated Goat Anti-Rabbit IgG(H+L).IP
IP result of anti-NRF2, NFE2L2 (IP:4ug; Detection:1:6000) with HeLa cells lysate 1500ug.| Product Name | NRF2, NFE2L2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human NRF2, NFE2L2 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:2000-1:12000 IHC:1:50-1:500 ICC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | Human |
| Concentration | 0.7mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFE2L2 |
|---|---|
| Gene Synonyms | NRF2 HEBP1 Nrf-2 IMDDHH |
| Gene Full Name | NFE2 like bZIP transcription factor 2 |
| Gene Summary | This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015] |
| Molecular Weight(MW) | 110 kDa, 68kDa |
| Cellular Localization | Cytosol;Nucleus |
WB
Various lysates were subjected to SDS PAGE followed by western blot with NRF2, NFE2L2 antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin embedded human liver cancer tissue slide using NRF2, NFE2L2 Antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NRF2, NFE2L2 antibody at dilution of 1:50 and CoraLite488 Conjugated Goat Anti-Rabbit IgG(H+L).
IP
IP result of anti-NRF2, NFE2L2 (IP:4ug; Detection:1:6000) with HeLa cells lysate 1500ug.| Application Notes | WB:1:2000-1:12000 IHC:1:50-1:500 ICC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Data sheet for OM643540