WB
Western blot analysis of lysates from 1)Hela cell , 2)Hela cells knockdown by NRF2-siRNA (F:CAUUGAUGUUUCUGAUCUATT,R:UAGAUCAGAAACAUCAAUGTT), (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody was diluted at 1:10000, 37° 1hour. (Red) GAPDH Monoclonal Antibody was diluted at 1:5000 as loading control, 4° over night, Dylight 680 secondary antibody was diluted at 1:10000, 37° 1hour.IHC
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Nrf2 Antibody. The picture on the right is blocked with the synthesized peptide.ICC/IF
Immunofluorescence analysis of HUVEC cells, using Nrf2 Antibody. The picture on the right is blocked with the synthesized peptide.| Product Name | Nrf2 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Nrf2. AA range:556-605. |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NFE2L2 |
|---|---|
| Gene Synonyms | NRF2 HEBP1 Nrf-2 IMDDHH |
| Gene Full Name | NFE2 like bZIP transcription factor 2 |
| Gene Summary | This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015] |
| Molecular Weight(MW) | 68kDa(Calculated),90-100kDa(Observed). |
| Cellular Localization | Cytoplasm, cytosol . Nucleus . Cytosolic under unstressed conditions: ubiquitinated and degraded by the BCR(KEAP1) E3 ubiquitin ligase complex (PubMed:15601839, PubMed:21196497). Translocates into the nucleus upon induction by electrophilic agents that inactivate the BCR(KEAP1) E3 ubiquitin ligase complex (PubMed:21196497). |
WB
Western blot analysis of lysates from 1)Hela cell , 2)Hela cells knockdown by NRF2-siRNA (F:CAUUGAUGUUUCUGAUCUATT,R:UAGAUCAGAAACAUCAAUGTT), (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody was diluted at 1:10000, 37° 1hour. (Red) GAPDH Monoclonal Antibody was diluted at 1:5000 as loading control, 4° over night, Dylight 680 secondary antibody was diluted at 1:10000, 37° 1hour.
IHC
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Nrf2 Antibody. The picture on the right is blocked with the synthesized peptide.
ICC/IF
Immunofluorescence analysis of HUVEC cells, using Nrf2 Antibody. The picture on the right is blocked with the synthesized peptide.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
Data sheet for OM644117