WB
Western blot analysis of lysates from Mouse lung using Claudin 5 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG(H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit ). Exposure time: 10s.IHC
Immunohistochemistry analysis of paraffin embedded Rat spleen tissue using Claudin 5 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.IP
Immunoprecipitation of Claudin 5 from 600ug extracts of Mouse lung tissue was performed using 3 µg of Claudin 5 Rabbit mAb. Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of i.mmunoprecipitates was conducted using Claudin 5 Rabbit mAb at a dilution of 1:1000.IHC
Immunohistochemistry analysis of paraffin embedded Mouse spleen tissue using Claudin 5 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Human lung squamous carcinoma tissue tissue using Claudin 5 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.| Product Name | Claudin 5 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 119-218 of human Claudin 5 (NP_001349995.1). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:1000-1:6000 IHC:1:800-1:8000 IP:0.5μg-4μg antibody for 400μg-800μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CLDN5 |
|---|---|
| Gene Synonyms | AWAL BEC1 TMVCF TMDVCF CPETRL1 |
| Gene Full Name | claudin 5 |
| Gene Summary | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018] |
| Alternative Names | AWAL BEC1 TMVCF TMDVCF CPETRL1 |
| Molecular Weight(MW) | 23kDa(Observed MW 20kDa) |
| Cellular Localization | Cell junction,Cell membrane,Multi-pass membrane protein,tight junction,apicolateral plasma membrane,bicellular tight junction,cell junction,cell-cell junction,lateral plasma membrane,plasma membrane. |
WB
Western blot analysis of lysates from Mouse lung using Claudin 5 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG(H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit ). Exposure time: 10s.
IHC
Immunohistochemistry analysis of paraffin embedded Rat spleen tissue using Claudin 5 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
IP
Immunoprecipitation of Claudin 5 from 600ug extracts of Mouse lung tissue was performed using 3 µg of Claudin 5 Rabbit mAb. Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of i.mmunoprecipitates was conducted using Claudin 5 Rabbit mAb at a dilution of 1:1000.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse spleen tissue using Claudin 5 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Human lung squamous carcinoma tissue tissue using Claudin 5 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.| Application Notes | WB:1:1000-1:6000 IHC:1:800-1:8000 IP:0.5μg-4μg antibody for 400μg-800μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3. |
