WB
Various lysates were subjected to SDS PAGE followed by western blot with NELFB antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin embedded human urothelial carcinoma tissue slide using NELFB antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of BxPC-3 cells using NELFB antibody at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).IP
IP result of anti-NELFB (IP:3ug; Detection:1:500) with mouse brain tissue lysate 6000ug.| Product Name | NELFB Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human NELF-B protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000-1:6000 IHC:1:200-1:1000 ICC:1:10-1:100|IP |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NELFB |
|---|---|
| Gene Synonyms | COBRA1 NELF-B |
| Gene Full Name | negative elongation factor complex member B |
| Gene Summary | NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008] |
| Alternative Names | COBRA1 Cofactor of BRCA1 KIAA1182 Negative elongation factor B NELF B |
| Molecular Weight(MW) | 66kDa |
| Cellular Localization | Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with NELFB antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin embedded human urothelial carcinoma tissue slide using NELFB antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of BxPC-3 cells using NELFB antibody at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP
IP result of anti-NELFB (IP:3ug; Detection:1:500) with mouse brain tissue lysate 6000ug.| Application Notes | WB:1:1000-1:6000 IHC:1:200-1:1000 ICC:1:10-1:100|IP |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
