WB
Western blot analysis of Peroxiredoxin 6 on different lysates with Rabbit anti-Peroxiredoxin 6 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: Mouse lung tissue lysate (40 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Rat lung tissue lysate (40 µg/Lane) Lane 6: Rat liver tissue lysate (40 µg/Lane) Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Peroxiredoxin 6 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Peroxiredoxin 6 aa 175-224 / 224. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRDX6 |
|---|---|
| Gene Synonyms | PRX p29 AOP2 1-Cys NSGPx aiPLA2 LPCAT-5 HEL-S-128m |
| Gene Full Name | peroxiredoxin 6 |
| Gene Summary | The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq, Jul 2008] |
| Alternative Names | 1 Cys antibody 1 Cys peroxiredoxin antibody 1 Cys PRX antibody 1 cysPrx antibody 1-Cys peroxiredoxin antibody 1-Cys PRX antibody 24 kDa protein antibody 9430088D19Rik antibody AA690119 antibody Acidic calcium independent phospholipase A2 antibody 1 Cys antibody 1 Cys peroxiredoxin antibody 1 Cys PRX antibody 1 cysPrx antibody 1-Cys peroxiredoxin antibody 1-Cys PRX antibody 24 kDa protein antibody 9430088D19Rik antibody AA690119 antibody Acidic calcium independent phospholipase A2 antibody Acidic calcium-independent phospholipase A2 antibody aiPLA2 antibody Antioxidant protein 2 antibody AOP2 antibody Aop2 rs3 antibody Brp 12 antibody Ciliary body glutathione peroxidase antibody CP 3 antibody EC 1.11.1.15 antibody EC 1.11.1.7 antibody EC 3.1.1. antibody Epididymis secretory sperm binding protein Li 128m antibody GPx antibody HEL S 128m antibody KIAA0106 antibody Liver 2D page spot 40 antibody Ltw4 antibody Lvtw 4 antibody MGC46173 antibody mKIAA0106 antibody Non selenium glutathione peroxidase antibody Non-selenium glutathione peroxidase antibody NSGPx antibody ORF06 antibody OTTHUMP00000032693 antibody p29 antibody Peroxiredoxin-6 antibody Peroxiredoxin6 antibody PHGPx antibody Phospholipase A2 lysosomal antibody PLA2 antibody PRDX 6 antibody Prdx5 antibody PRDX6 antibody Prdx6 rs3 antibody PRDX6_HUMAN antibody PRX antibody Red blood cells page spot 12 antibody Thiol specific antioxidant protein antibody |
| Molecular Weight(MW) | 25kDa(Observed band size: 27kDa) |
| Cellular Localization | Cytoplasm. Lysosome. |
WB
Western blot analysis of Peroxiredoxin 6 on different lysates with Rabbit anti-Peroxiredoxin 6 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: Mouse lung tissue lysate (40 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Rat lung tissue lysate (40 µg/Lane) Lane 6: Rat liver tissue lysate (40 µg/Lane) Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
