WB
Western blot analysis of Androgen receptor on different lysates with Rabbit anti-Androgen receptor antibody at 1/1,000 dilution. Lane 1: A549 cell lysate, Lane 2: K-562 cell lysate, Lane 3: 22RV1 cell lysate, Lysates/proteins at 10 µg/Lane. Predicted band size: 99 kDa Observed band size: 99/75 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of Androgen receptor on rat testis tissue lysates with Rabbit anti-Androgen receptor antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 99 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-Androgen receptor antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of 22RV1 cells labeling Androgen receptor with Rabbit anti-Androgen receptor antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Androgen receptor antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.IF-P
Immunofluorescence analysis of paraffin-embedded rat epididymis tissue labeling Androgen receptor with Rabbit anti-Androgen receptor antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).IP
Androgen receptor was immunoprecipitated from 0.2 mg 22RV1 cell lysate with Rabbit anti-Androgen receptor antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Androgen receptor antibody at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 22RV1 cell lysate (input), Lane 2: Rabbit anti-Androgen receptor antibody IP in 22RV1 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Androgen receptor antibody in 22RV1 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 25 seconds.| Product Name | Androgen receptor Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Androgen Receptor aa 300-400 (N terminal). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCIPWB |
| WB:1:1000 IHC:1:200-1:1000 ICC:1:100 IF-P:1:50-1:200 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | AR |
|---|---|
| Gene Synonyms | KD AIS AR8 TFM DHTR SBMA HYSP1 NR3C4 SMAX1 HUMARA |
| Gene Full Name | androgen receptor |
| Gene Summary | The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017] |
| Alternative Names | AIS antibody ANDR_HUMAN antibody Androgen nuclear receptor variant 2 antibody Androgen receptor (dihydrotestosterone receptor testicular feminization spinal and bulbar muscular atrophy Kennedy disease) antibody Androgen receptor antibody androgen receptor splice variant 4b antibody AR antibody AIS antibody ANDR_HUMAN antibody Androgen nuclear receptor variant 2 antibody Androgen receptor (dihydrotestosterone receptor testicular feminization spinal and bulbar muscular atrophy Kennedy disease) antibody Androgen receptor antibody androgen receptor splice variant 4b antibody AR antibody AR8 antibody DHTR antibody Dihydro testosterone receptor antibody Dihydrotestosterone receptor (DHTR) antibody Dihydrotestosterone receptor antibody HUMARA antibody HYSP1 antibody KD antibody Kennedy disease (KD) antibody NR3C4 antibody Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody Nuclear receptor subfamily 3 group C member 4 antibody SBMA antibody SMAX1 antibody Spinal and bulbar muscular atrophy (SBMA) antibody Spinal and bulbar muscular atrophy antibody Testicular Feminization (TFM) antibody TFM antibody |
| Molecular Weight(MW) | 99kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of Androgen receptor on different lysates with Rabbit anti-Androgen receptor antibody at 1/1,000 dilution. Lane 1: A549 cell lysate, Lane 2: K-562 cell lysate, Lane 3: 22RV1 cell lysate, Lysates/proteins at 10 µg/Lane. Predicted band size: 99 kDa Observed band size: 99/75 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of Androgen receptor on rat testis tissue lysates with Rabbit anti-Androgen receptor antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 99 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-Androgen receptor antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of 22RV1 cells labeling Androgen receptor with Rabbit anti-Androgen receptor antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Androgen receptor antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
IF-P
Immunofluorescence analysis of paraffin-embedded rat epididymis tissue labeling Androgen receptor with Rabbit anti-Androgen receptor antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
IP
Androgen receptor was immunoprecipitated from 0.2 mg 22RV1 cell lysate with Rabbit anti-Androgen receptor antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Androgen receptor antibody at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 22RV1 cell lysate (input), Lane 2: Rabbit anti-Androgen receptor antibody IP in 22RV1 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Androgen receptor antibody in 22RV1 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 25 seconds.| Application Notes | WB:1:1000 IHC:1:200-1:1000 ICC:1:100 IF-P:1:50-1:200 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
