WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Androgen Receptor antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: LnCap Lane 2: MCF7 Lane 2: mouse testis .IHC
Human prostate carcinoma was stained with anti-Androgen Receptor rabbit antibody.ICC/IF
Immunofluorescence analysis of Hela cell. 1,AR Monoclonal Antibody(red) was diluted at 1:200(4° overnight). GFAP Monoclonal Antibody(5C8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).| Product Name | Androgen Receptor Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 ICC/IF:1:200-1:1000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | AR |
|---|---|
| Gene Synonyms | KD AIS AR8 TFM DHTR SBMA HYSP1 NR3C4 SMAX1 HUMARA |
| Gene Full Name | androgen receptor |
| Gene Summary | The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017] |
| Alternative Names | Androgen receptor Dihydrotestosterone receptor Nuclear receptor subfamily 3 group C member 4 |
| Molecular Weight(MW) | 99kDa(Observed MW 110kDa) |
| Cellular Localization | Nuclear |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Androgen Receptor antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: LnCap Lane 2: MCF7 Lane 2: mouse testis .
IHC
Human prostate carcinoma was stained with anti-Androgen Receptor rabbit antibody.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,AR Monoclonal Antibody(red) was diluted at 1:200(4° overnight). GFAP Monoclonal Antibody(5C8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).| Application Notes | WB:1:2000-1:10000 ICC/IF:1:200-1:1000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
