WB
Various lysates were subjected to SDS PAGE followed by western blot with androgen receptor antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using androgen receptor antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using androgen receptor antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).IF-P
Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using androgen receptor antibody at dilution of 1:200 andOmnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Product Name | androgen receptor Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human AR protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:5000-1:50000 IHC:1:200-1:800 ICC/IF:1:200-1:800 IF-P:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.85mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AR |
|---|---|
| Gene Synonyms | KD AIS AR8 TFM DHTR SBMA HYSP1 NR3C4 SMAX1 HUMARA |
| Gene Full Name | androgen receptor |
| Gene Summary | The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017] |
| Alternative Names | AR androgen receptor AR NR3C4 DHTR |
| Molecular Weight(MW) | 75-80 kDa, 110-120 kDa. |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with androgen receptor antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using androgen receptor antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using androgen receptor antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).
IF-P
Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using androgen receptor antibody at dilution of 1:200 andOmnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Application Notes | WB:1:5000-1:50000 IHC:1:200-1:800 ICC/IF:1:200-1:800 IF-P:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
