WB
Western blot analysis of various lysates using α-Synuclein Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): T-47D Exposure time: 90s.IHC
Immunohistochemistry analysis of paraffin embedded Rat brain tissue using α-Synuclein Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.ICC/IF
Confocal imaging of HEL cells using α Synuclein Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). objective: 100x.IP
Immunoprecipitation of α-Synuclein in 500 µg extracts from HEL cells using 2 µg α-Synuclein Rabbit mAb. Western blot analysis was performed using α-Synuclein Rabbit mAb at 1:3000 dilution.| Product Name | α-Synuclein Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human α-Synuclein(NP_000336.1). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000-1:2000 IHC:1:200-1:2000 ICC:1:200-1:800 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNCA |
|---|---|
| Gene Synonyms | PD1 NACP PARK1 PARK4 |
| Gene Full Name | synuclein alpha |
| Gene Summary | Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016] |
| Alternative Names | PD1 NACP PARK1 PARK4 α-Synuclein |
| Molecular Weight(MW) | 14kDa(Observed MW 18kDa) |
WB
Western blot analysis of various lysates using α-Synuclein Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): T-47D Exposure time: 90s.
IHC
Immunohistochemistry analysis of paraffin embedded Rat brain tissue using α-Synuclein Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ICC/IF
Confocal imaging of HEL cells using α Synuclein Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). objective: 100x.
IP
Immunoprecipitation of α-Synuclein in 500 µg extracts from HEL cells using 2 µg α-Synuclein Rabbit mAb. Western blot analysis was performed using α-Synuclein Rabbit mAb at 1:3000 dilution.| Application Notes | WB:1:1000-1:2000 IHC:1:200-1:2000 ICC:1:200-1:800 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
