IHC
Formalin-Fixed, Paraffin-embedded Breast carcinoma stained with pan Cytokeratin Antibody| Product Name | pan Cytokeratin Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Human epidermal keratin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:0.5-1 µg/ml. IHC:0.5-1 µg/ml for 30 minutes at RT. ICC:1-2 µg/ml. |
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| Species Reactivity | HumanRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target Description | Twenty Human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI < 5.7) and basic (pI > 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. This Monoclonal Antibody is a broad spectrum anti pan-Cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains Cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas. |
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| Alternative Names | CK-1B Cytokeratin-1B K1B K77 Keratin type II cytoskeletal 1b Keratin-77 Keratin 1B KRT1B Type-II Keratin Kb39 |
| Cellular Localization | Cytoplasmic. |
IHC
Formalin-Fixed, Paraffin-embedded Breast carcinoma stained with pan Cytokeratin Antibody| Application Notes | WB:0.5-1 µg/ml. IHC:0.5-1 µg/ml for 30 minutes at RT. ICC:1-2 µg/ml. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store undiluted at 2-8°C. |
| Storage Buffer | 10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide |
