WB
Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with HA-tagged TrpV1 or untagged Kv2.1 plasmids and probed with N221/17 (left panel) or N52A/42 (right panel) TC supe.ICC/IF
Immunofluorescence staining of adult dorsal root ganglion sections from TrpV1 WT and KO -/- mice with N221/17 TC supe (red), Kv1.2 rabbit polyclonal (green) and Hoechst nuclear stain (blue).| Product Name | Trpv1 Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Fusion protein amino acids 1-160 (cytoplasmic N-terminus) of rat TrpV1 (also known as Transient receptor potential cation channel subfamily V member 1, Osm-9-like TRP channel 1, OTRPC1, Capsaicin receptor, Vanilloid receptor 1, VR1, Vanilloid receptor type |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Trpv1 |
|---|---|
| Gene Synonyms | Vr1 VR-1 OTRPC1 TRPV1beta TRPV1alpha |
| Gene Full Name | transient receptor potential cation channel, subfamily V, member 1 |
| Gene Summary | Enables calcium channel activity; identical protein binding activity; and temperature-gated ion channel activity. Involved in detection of temperature stimulus involved in sensory perception of pain; monoatomic ion transmembrane transport; and negative regulation of transcription by RNA polymerase II. Acts upstream of or within several processes, including diet induced thermogenesis; fever generation; and sensory perception of chemical stimulus. Located in neuron projection and plasma membrane. Is active in GABA-ergic synapse and postsynaptic membrane. Is expressed in central nervous system; cochlea; dorsal root ganglion; and eye. Human ortholog(s) of this gene implicated in migraine. Orthologous to human TRPV1 (transient receptor potential cation channel subfamily V member 1). [provided by Alliance of Genome Resources, Jan 2025] |
| Molecular Weight(MW) | 100kDa |
| Cellular Localization | Cell membrane |
WB
Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with HA-tagged TrpV1 or untagged Kv2.1 plasmids and probed with N221/17 (left panel) or N52A/42 (right panel) TC supe.
ICC/IF
Immunofluorescence staining of adult dorsal root ganglion sections from TrpV1 WT and KO -/- mice with N221/17 TC supe (red), Kv1.2 rabbit polyclonal (green) and Hoechst nuclear stain (blue).| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
