WB
Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti- C2orf43 at 1/1000 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 35 kDa.IHC
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C2orf43) Polyclonal Antibody at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.| Product Name | C2orf43 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human C2orf43: 1-100/325 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LDAH |
|---|---|
| Gene Synonyms | hLDAH C2orf43 |
| Gene Full Name | lipid droplet associated hydrolase |
| Gene Summary | Predicted to enable sterol ester esterase activity. Involved in cholesterol homeostasis and intracellular triglyceride homeostasis. Acts upstream of with a positive effect on lipid droplet fusion. Located in endoplasmic reticulum and lipid droplet. [provided by Alliance of Genome Resources, Feb 2025] |
| Alternative Names | C2orf43 hLDAH LDAH_HUMAN LDAH Lipid droplet-associated serine hydrolase (hLDAH) 3.1.1.13 lipid droplet associated hydrolase chromosome 2 open reading frame 43 |
| Molecular Weight(MW) | 37kDa |
| Cellular Localization | Lipid droplet,Endoplasmic reticulum. |
WB
Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti- C2orf43 at 1/1000 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 35 kDa.
IHC
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C2orf43) Polyclonal Antibody at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
