WB
Western blot analysis of anti-C2orf43/LDAH antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human SK-N-SH whole cell lysates, Lane 7: human SH-SY5Y whole cell lysates, Lane 8: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-C2orf43/LDAH antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.WB
Western blot analysis of anti-C2orf43/LDAH antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat RH-35 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-C2orf43/LDAH antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
C2orf43/LDAH was detected in a paraffin-embedded section of human glioma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
C2orf43/LDAH was detected in a paraffin-embedded section of rat alcoholic liver tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.FC
Overlay histogram showing Jurkat cells stained with rabbit anti-C2orf43/LDAH Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C2orf43/LDAH Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to C2orf43/LDAH |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human LDAH recombinant protein (Position: E7-D281). Human LDAH shares 68.8% and 68.5% amino acid (aa) sequence identity with mouse and rat LDAH, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LDAH |
|---|---|
| Gene Synonyms | hLDAH C2orf43 |
| Gene Full Name | lipid droplet associated hydrolase |
| Gene Summary | Predicted to enable sterol ester esterase activity. Involved in cholesterol homeostasis and intracellular triglyceride homeostasis. Acts upstream of with a positive effect on lipid droplet fusion. Located in endoplasmic reticulum and lipid droplet. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | C2orf43 hLDAH |
| Molecular Weight(MW) | 37 kDa |
| Cellular Localization | Endoplasmic reticulum,Lipid droplet. |
WB
Western blot analysis of anti-C2orf43/LDAH antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human SK-N-SH whole cell lysates, Lane 7: human SH-SY5Y whole cell lysates, Lane 8: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-C2orf43/LDAH antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
WB
Western blot analysis of anti-C2orf43/LDAH antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat RH-35 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-C2orf43/LDAH antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
C2orf43/LDAH was detected in a paraffin-embedded section of human glioma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
C2orf43/LDAH was detected in a paraffin-embedded section of rat alcoholic liver tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
FC
Overlay histogram showing Jurkat cells stained with rabbit anti-C2orf43/LDAH Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C2orf43/LDAH Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
