WB
Western blot analysis of Active+Pro Caspase-9 on different lysates with Rabbit anti-Active+Pro Caspase-9 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate， Lane 2: HeLa treated with 1μM staurosporine for 4 hours cell lysate， Lane 3: HeLa treated with 3μM staurosporine for 4 hours cell lysate， Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Active+Pro Caspase-9 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human Caspase-9 aa 95-335 / 416. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:500-1:2000 IP:Use at an assay dependent concentration. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CASP9 |
|---|---|
| Gene Synonyms | MCH6 APAF3 APAF-3 PPP1R56 ICE-LAP6 |
| Gene Full Name | caspase 9 |
| Gene Summary | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013] |
| Alternative Names | Caspase9 APAF-3 antibody APAF3 antibody Apoptosis related cysteine peptidase antibody Apoptotic protease Mch-6 antibody Apoptotic protease-activating factor 3 antibody CASP-9 antibody CASP9 antibody CASP9_HUMAN antibody Caspase 9 apoptosis related cysteine peptidase antibody Caspase9 APAF-3 antibody APAF3 antibody Apoptosis related cysteine peptidase antibody Apoptotic protease Mch-6 antibody Apoptotic protease-activating factor 3 antibody CASP-9 antibody CASP9 antibody CASP9_HUMAN antibody Caspase 9 apoptosis related cysteine peptidase antibody Caspase 9 Dominant Negative antibody Caspase 9c antibody Caspase-9 antibody Caspase-9 subunit p10 antibody ICE LAP6 antibody ICE like apoptotic protease 6 antibody ICE-LAP6 antibody ICE-like apoptotic protease 6 antibody MCH6 antibody PPP1R56 antibody protein phosphatase 1 regulatory subunit 56 antibody RNCASP9 antibody |
| Molecular Weight(MW) | 46kDa(Observed band size: 46/37/35kDa) |
| Cellular Localization | Apoptosome, cytosol, Mitochondrion, nucleus, cytoplasm, protein-containing complex. |
WB
Western blot analysis of Active+Pro Caspase-9 on different lysates with Rabbit anti-Active+Pro Caspase-9 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate， Lane 2: HeLa treated with 1μM staurosporine for 4 hours cell lysate， Lane 3: HeLa treated with 3μM staurosporine for 4 hours cell lysate， Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:500-1:2000 IP:Use at an assay dependent concentration. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
