ICC/IF
Immunocytochemistry analysis of HT-29 (positive) and MDA-MB-231 (negative) cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody at 1/2,000 dilution.IF-P
Immunofluorescence analysis of paraffin-embedded human colon tissue labeling E-Cadherin with Rabbit anti-E-Cadherin antibody at 1/50 dilution.FC
Flow cytometric analysis of HT-29 (positive, red) and MDA-MB-231 (negative, green) cells labeling E-Cadherin.| Product Name | E-Cadherin Recombinant Rabbit Monoclonal Antibody-AF488 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human E-Cadherin aa 580-630 (Extracellular). |
| Conjugation | AF488 |
| Isotype | IgG |
|---|---|
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-P |
| ICC/IF:1:2000 IF-P:1:50-1:200 FC:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CDH1 |
|---|---|
| Gene Synonyms | UVO CDHE ECAD LCAM Arc-1 BCDS1 CD324 |
| Gene Full Name | cadherin 1 |
| Gene Summary | A large family of ATPases has been described, whose key feature is that they share a conserved region of about 220 amino acids that contains an ATP-binding site. The proteins that belong to this family either contain one or two AAA (ATPases Associated with diverse cellular Activities) domains. AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes. The protein encoded by this gene contains two AAA domains, as well as a bromodomain. [provided by RefSeq, Jul 2008] |
| Alternative Names | Arc 1 antibody CADH1_HUMAN antibody Cadherin 1 antibody cadherin 1 type 1 E-cadherin antibody Cadherin1 antibody CAM 120/80 antibody CD 324 antibody CD324 antibody CD324 antigen antibody cdh1 antibody Arc 1 antibody CADH1_HUMAN antibody Cadherin 1 antibody cadherin 1 type 1 E-cadherin antibody Cadherin1 antibody CAM 120/80 antibody CD 324 antibody CD324 antibody CD324 antigen antibody cdh1 antibody CDHE antibody E-Cad/CTF3 antibody E Cadherin antibody E-cadherin antibody ECAD antibody Epithelial cadherin antibody epithelial calcium dependant adhesion protein antibody LCAM antibody Liver cell adhesion molecule antibody UVO antibody Uvomorulin antibody |
| Molecular Weight(MW) | 97kDa(Observed band size:80-120kDa) |
| Cellular Localization | Cell junction, Cell membrane, Endosome, Golgi apparatus. |
ICC/IF
Immunocytochemistry analysis of HT-29 (positive) and MDA-MB-231 (negative) cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody at 1/2,000 dilution.
IF-P
Immunofluorescence analysis of paraffin-embedded human colon tissue labeling E-Cadherin with Rabbit anti-E-Cadherin antibody at 1/50 dilution.
FC
Flow cytometric analysis of HT-29 (positive, red) and MDA-MB-231 (negative, green) cells labeling E-Cadherin.| Application Notes | ICC/IF:1:2000 IF-P:1:50-1:200 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
