WB
Western blot analysis of lysates from A-431 cells, using Phospho-Fyn-S21 Rabbit pAb at 1:1000 dilution. A-431 cells were treated by 10% FBS after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.| Product Name | Phospho-Fyn-S21 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic phosphorylated peptide around S21 of human Phospho-Fyn-S21 (NP_002028.1). |
| Modification | p-S21 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FYN |
|---|---|
| Gene Synonyms | SLK SYN p59-FYN |
| Gene Full Name | FYN proto-oncogene, Src family tyrosine kinase |
| Gene Summary | This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 61kDa |
| Cellular Localization | Cell membrane, Cytoplasm, Nucleus. |
WB
Western blot analysis of lysates from A-431 cells, using Phospho-Fyn-S21 Rabbit pAb at 1:1000 dilution. A-431 cells were treated by 10% FBS after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
Data sheet for OM644065