WB
Western blot analysis of extracts from various samples, using BCL7C Mouse Monoclonal Antibody at 1/1000 dilution. Lane 1: Mouse brain. Lane 2: Mouse spleen. Lane 3: Mouse kidney. Lane 4: Mouse lung.Exposure for 1min.IHC
BCL7C Mouse Monoclonal Antibody at 1/100 staining human lung cancer and para-carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.ICC/IF
BCL7C Mouse Monoclonal Antibody staining Hela cells by ICC/IF. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and rabbit anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-mouse IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | Mouse monoclonal antibody to BCL7C |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human BCL7C(Accession Q8WUZ0), corresponding to amino acid residues R75-V125. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:3000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCL7C |
|---|---|
| Gene Synonyms | SMARCJ3 |
| Gene Full Name | BAF chromatin remodeling complex subunit BCL7C |
| Gene Summary | This gene is identified by the similarity of its product to the N-terminal region of BCL7A protein. The BCL7A protein is encoded by the gene known to be directly involved in a three-way gene translocation in a Burkitt lymphoma cell line. The function of this gene has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013] |
| Alternative Names | B cell CLL/lymphoma 7 protein family member C B cell CLL/lymphoma 7C B-cell CLL/lymphoma 7 protein family member C BCL 7C Bcl7c BCL7C_HUMAN |
| Molecular Weight(MW) | 23kDa |
WB
Western blot analysis of extracts from various samples, using BCL7C Mouse Monoclonal Antibody at 1/1000 dilution. Lane 1: Mouse brain. Lane 2: Mouse spleen. Lane 3: Mouse kidney. Lane 4: Mouse lung.Exposure for 1min.
IHC
BCL7C Mouse Monoclonal Antibody at 1/100 staining human lung cancer and para-carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.
ICC/IF
BCL7C Mouse Monoclonal Antibody staining Hela cells by ICC/IF. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and rabbit anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-mouse IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:500-1:3000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
