WB
Western blot analysis of various lysates using TROP-2 Rabbit mAb at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.IHC
Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using TROP-2 Rabbit mAb at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Human pancreas tissue using TROP-2 Rabbit mAb at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.ICC/IF
Confocal imaging of MCF7 cells using TROP-2 Rabbit mAb (dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.| Product Name | TROP-2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 27-274 of human TROP-2 (NP_002344.2). |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:3000-1:9000 IHC:1:3000-1:12000 ICC/IF: 1:200-1:400 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TACSTD2 |
|---|---|
| Gene Synonyms | EGP1 GP50 M1S1 EGP-1 TROP2 GA7331 GA733-1 |
| Gene Full Name | tumor associated calcium signal transducer 2 |
| Gene Summary | This intronless gene encodes a carcinoma-associated antigen. This antigen is a cell surface receptor that transduces calcium signals. Mutations of this gene have been associated with gelatinous drop-like corneal dystrophy.[provided by RefSeq, Dec 2009] |
| Alternative Names | EGP1 GP50 M1S1 EGP-1 TROP2 GA7331 GA733-1 TROP-2 |
| Molecular Weight(MW) | 36kDa（Observed MW 40-65kDa） |
| Cellular Localization | Membrane, Single-pass type I membrane protein. |
WB
Western blot analysis of various lysates using TROP-2 Rabbit mAb at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
IHC
Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using TROP-2 Rabbit mAb at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Human pancreas tissue using TROP-2 Rabbit mAb at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ICC/IF
Confocal imaging of MCF7 cells using TROP-2 Rabbit mAb (dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.| Application Notes | WB:1:3000-1:9000 IHC:1:3000-1:12000 ICC/IF: 1:200-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
