WB
Western blot analysis of TROP2 on different lysates with Rabbit anti-TROP2 antibody at 1/5,000 dilution. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature. Negative control: HEK-293.WB
Western blot analysis of TROP2 on different lysates with Rabbit anti-TROP2 antibody at 1/5,000 dilution. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature. Negative control: liver tissue.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-TROP2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TROP2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-TROP2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of MCF7 (positive) and HEK-293 (negative) labeling TROP2 with Rabbit anti-TROP2 antibody at 1/2,500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TROP2 antibody at 1/2,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution. Negative control: HEK-293.IF-F
Application: IHC-Fr,Species: Mouse,Site: kidney,Sample: Frozen section,Antibody concentration: 1/500,Antigen retrieval: Not required.IF-P
Species: Human,Site: skin,Sample: Paraffin-embedded section,Antibody concentration: 1/400.IF-P
Species: Rat,Site: skin,Sample: Paraffin-embedded section,Antibody concentration: 1/400.| Product Name | TROP2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-FIF-PIHCWB |
| WB:1:2000-1:5000 IHC:1:1000 ICC/IF:1:2500 IF-P:1:400 IF-F:1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TACSTD2 |
|---|---|
| Gene Synonyms | EGP1 GP50 M1S1 EGP-1 TROP2 GA7331 GA733-1 |
| Gene Full Name | tumor associated calcium signal transducer 2 |
| Gene Summary | This intronless gene encodes a carcinoma-associated antigen. This antigen is a cell surface receptor that transduces calcium signals. Mutations of this gene have been associated with gelatinous drop-like corneal dystrophy.[provided by RefSeq, Dec 2009] |
| Alternative Names | Cell surface glycoprotein Trop 2 antibody Cell surface glycoprotein Trop-2 antibody Cell surface glycoprotein Trop2 antibody Epithelial glycoprotein 1 antibody GA733 1 antibody GA7331 antibody M1S 1 antibody M1S1 antibody Membrane component chromosome 1 surface marker 1 antibody Pancreatic carcinoma marker protein GA733 1 antibody Cell surface glycoprotein Trop 2 antibody Cell surface glycoprotein Trop-2 antibody Cell surface glycoprotein Trop2 antibody Epithelial glycoprotein 1 antibody GA733 1 antibody GA7331 antibody M1S 1 antibody M1S1 antibody Membrane component chromosome 1 surface marker 1 antibody Pancreatic carcinoma marker protein GA733 1 antibody Pancreatic carcinoma marker protein GA733-1 antibody Pancreatic carcinoma marker protein GA7331 antibody TACD 2 antibody TACD2_HUMAN antibody TACSTD 2 antibody Tacstd2 antibody Trop 2 antibody Trop2 antibody Tumor associated calcium signal transducer 2 precursor antibody Tumor-associated calcium signal transducer 2 antibody |
| Molecular Weight(MW) | 36 kDa(Observed band size: 40-60 kDa) |
| Cellular Localization | Membrane. |
WB
Western blot analysis of TROP2 on different lysates with Rabbit anti-TROP2 antibody at 1/5,000 dilution. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature. Negative control: HEK-293.
WB
Western blot analysis of TROP2 on different lysates with Rabbit anti-TROP2 antibody at 1/5,000 dilution. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature. Negative control: liver tissue.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-TROP2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TROP2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-TROP2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of MCF7 (positive) and HEK-293 (negative) labeling TROP2 with Rabbit anti-TROP2 antibody at 1/2,500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TROP2 antibody at 1/2,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution. Negative control: HEK-293.
IF-F
Application: IHC-Fr,Species: Mouse,Site: kidney,Sample: Frozen section,Antibody concentration: 1/500,Antigen retrieval: Not required.
IF-P
Species: Human,Site: skin,Sample: Paraffin-embedded section,Antibody concentration: 1/400.
IF-P
Species: Rat,Site: skin,Sample: Paraffin-embedded section,Antibody concentration: 1/400.| Application Notes | WB:1:2000-1:5000 IHC:1:1000 ICC/IF:1:2500 IF-P:1:400 IF-F:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
