WB
Western Blot analysis of 22RV1 cells using AR-β3 Polyclonal Antibody diluted at 1:1000.WB
Western blot analysis of lysates from K562 cells, using ADRB3 Antibody. The lane on the right is blocked with the synthesized peptide.ICC/IF
Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, DAPI(blue) 10min.| Product Name | AR-β3 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ADRB3. AA range:250-299. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-2000 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ADRB3 |
|---|---|
| Gene Synonyms | BETA3AR |
| Gene Full Name | adrenoceptor beta 3 |
| Gene Summary | The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor is located mainly in the adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Obesity and bodyweight-related disorders are correlated with certain polymorphisms in three subtypes of beta-adrenoceptor, among them, the ADRB3 gene.[provided by RefSeq, Oct 2019] |
| Alternative Names | ADRB3 ADRB3R B3AR Beta-3 adrenergic receptor Beta-3 adrenoreceptor Beta-3 adrenoceptor |
| Molecular Weight(MW) | 36kDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
WB
Western Blot analysis of 22RV1 cells using AR-β3 Polyclonal Antibody diluted at 1:1000.
WB
Western blot analysis of lysates from K562 cells, using ADRB3 Antibody. The lane on the right is blocked with the synthesized peptide.
ICC/IF
Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, DAPI(blue) 10min.| Application Notes | WB:1:500-2000 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
