WB
Primary: Anti-ADRB3 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.IHC
Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-ADRB3 Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining.IHC
Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-ADRB3 Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining| Product Name | ADRB3 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human ADRB3 : 51-150/408 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ADRB3 |
|---|---|
| Gene Synonyms | BETA3AR |
| Gene Full Name | adrenoceptor beta 3 |
| Gene Summary | The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor is located mainly in the adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Obesity and bodyweight-related disorders are correlated with certain polymorphisms in three subtypes of beta-adrenoceptor, among them, the ADRB3 gene.[provided by RefSeq, Oct 2019] |
| Alternative Names | BETA3AR Adrb-3 beta 3-AR ADRB ADRB3_HUMAN ADRB3 Beta-3 adrenoreceptor (Beta-3 adrenoceptor) ADRB3R B3AR ADRB3_MOUSE B3bar ADRB3_RAT adrenoceptor beta 3 adrenergic beta-3- receptor |
| Molecular Weight(MW) | 44 kDa(Observed band size: 48 kDa) |
| Cellular Localization | Cell membrane,Membrane. |
| SwissProt ID | P13945 |
|---|
WB
Primary: Anti-ADRB3 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.
IHC
Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-ADRB3 Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining.
IHC
Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-ADRB3 Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
