WB
Western blot analysis of ADRB3 using anti-ADRB3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ADRB3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of ADRB3 using anti-ADRB3 antibody. ADRB3 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-ADRB3 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody to ADRB3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human beta 3 Adrenergic Receptor/ADRB3 recombinant protein (Position: R179-S250). Human ADRB3 shares 88.9% and 91.7% amino acid (aa) sequence identity with mouse and rat ADRB3, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADRB3 |
|---|---|
| Gene Synonyms | BETA3AR |
| Gene Full Name | adrenoceptor beta 3 |
| Gene Summary | The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor is located mainly in the adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Obesity and bodyweight-related disorders are correlated with certain polymorphisms in three subtypes of beta-adrenoceptor, among them, the ADRB3 gene.[provided by RefSeq, Oct 2019] |
| Alternative Names | adrenoceptor beta 3 BETA3AR ADRB3 ADRB3R |
| Molecular Weight(MW) | 44 kDa |
| Cellular Localization | Cell membrane,Membrane. |
WB
Western blot analysis of ADRB3 using anti-ADRB3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ADRB3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of ADRB3 using anti-ADRB3 antibody. ADRB3 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-ADRB3 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
